Table 2.

Oligonucleotides Used. The Nonhomologous Tail in the S1 Mapping Primer S1-04nht Is Underlined

Tn5062 linker oligonucleotides
VC1	5′-GATCTGAATTCGGATCCTAATTAATTAATCTAGAAAGGAGGTGATCA
VC2	5′-TATGATCACCTCCTTTCTAGATTAATTAATTAGGATCCGAATTCA
VC3	5′-TATGGACGGAGCTCGGCCGCTTAAGGTACCGAATTCC
VC4	5′-TCGAGGAATTCGGTACCTTAAGCGGCCGAGCTCCGTCCA

Tn5062 sequencing primers
EZR1	5′-ATGCGCTCCATCAAGAAGAG
EZL2	5′-TCCAGCTCGACCAGGATG

IPCR primers
Tsr1	5′-GCAAGGGGAAGTCGTCGCTCTCTGG
Tsr2	5′-CGGCGTGGGGACGTCGTCGTTCTCG
Tsr3	5′-GGATTTGCGATGGTGTCCAACTCAG
Tsr4	5′-GGACGTCGAGCCCCTCATGCACAGC
Tsr5	5′-GGAGAGAACGACGGGAAGGGAGAAG
Tsr6	5′-GACCTCGATTCGTCAGTGATGATCC
Tsr7	5′-CGGATTGTCCCCGAGTTCCTTCACG
Tsr8	5′-GCGTCTTCCGCCTCGGTTTCCATCC

S1 mapping primers
S1-04nht	5′-GCGCAAGCTTCCCGAAGCCGTGTAGAAGTA
S1-04r	5′-AGTGCTTTGAGCGCTTCCT