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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochimie. 2015 Apr 14;114:30–38. doi: 10.1016/j.biochi.2015.04.001

Figure 3.

Figure 3

Single-molecule FRET measurements of 30S-bound IF3. Cartoon representations (top row) of various 30S ICs including (a) a 30S IC lacking a tRNA, (b) a canonical 30S IC carrying fMet-tRNAfMet base paired to the AUG start codon, (c) a pseudo 30S IC carrying Phe-tRNAPhe, and (d) a non-canonical 30S IC assembled on the AUU near-start codon. Surface contour plots (bottom row) of the time-evolution of population FRET were constructed for each 30S IC by superimposing all of the individual EFRET versus time trajectories obtained (not shown), which is given by the ‘N’ value. Normalized one-dimensional EFRET histograms are shown to the right of each surface contour plot. Surface contour plots are plotted from the lowest (tan) to the highest population (red) as indicated by the population color bar. Figure adapted from Elvekrog et al.59.