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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Acta Neuropathol. 2016 Jan 4;131(4):605–620. doi: 10.1007/s00401-015-1530-0

Figure 6. Pur-alpha co-expression rescues mutant FUS mislocalization in primary motor neurons.

Figure 6

(a) Representative confocal images of primary motor neurons expressing GFP-FUS-WT and GFP-FUS-R521G alone or coexpressed with mCherry-Pur-alpha. Cytoplasmic mislocalization of FUS-R521G was evident in motor neurons whereas coexpression with Pur-alpha prevented this mislocalization. (b) Bar graph shows the mean percentage of neurons with FUS localized in the nucleus alone or nucleus + cytoplasm under different condition. The percentage of neurons with FUS-R521G in the nucleus alone was significantly increased upon co-expression with Pur-alpha. Student T test were prerformed. Error bars represent ± SEM. (**P=0.01) Scale= 10 μm.