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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Sep 14;35(17):2247–2265. doi: 10.1038/onc.2015.288

Figure 6. YAP stimulates expression FGF and FGFR and secretion of basic and acidic FGFs.

Figure 6

a) Determining the mRNA expression of YAP, FGF ligands (FGF1&FGF2) and FGFR1-4 in FT194-MXIV, FT194-YAP, and FT194-YAPS127A cells (Left panel), and FT246-MXIV, FT246-YAP and FT246-YAPS127A cells (right panels) by RT-PCR. AREG was used as a positive control. GAPDH was used as a loading control. b) Verteporfin (VTPF) suppresses YAP–induced production of basic FGF (FGF2). Left panel: FGF2 levels in the cell lysate of FT194-MXIV, FT194-YAP and FT194-YAPS127A cells in the absence or presence of 5μM of VTPF. Right panel: FGF2 levels in the culture medium of FT194-MXIV, FT194-YAP, and FT194-YAPS127A cells in the absence or presence of 5μM of VTPF. c) Verteporfin (VTPF) suppresses YAP–induced production acidic FGF (FGF1). Left penal: FGF1 levels in the cell lysate of FT194-MXIV, FT194-YAP, and FT194-YAPS127A cells in the absence or presence of 5μM of VTPF. Right panel: FGF1 levels in the culture medium of FT194-MXIV, FT194-YAP and FT194-YAPS127A cells in the absence or presence of 5μM of VTPF. Each bar represents the mean ± SEM of at least three independent experiments. Bars with different letters are significantly different from each other (P < 0.05).