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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Sep 14;35(17):2247–2265. doi: 10.1038/onc.2015.288

Figure 9. The Hippo pathway is actively involved in the interaction between YAP and FGFR signaling pathway in the FTSECs.

Figure 9

a) FGF2 suppresses the phosphorylation of the major components of the Hippo/YAP signaling pathways in FT194 cells. The total and phosphorylated proteins were determined by Western blot. b) Western blot analysis indicates that knockdown of LATS1/2 in FT194 cells using LATS1/2 specific siRNA activates YAP, AKT (S473) and ERK1/2. c) RT-PCR results showing that knockdown of LATS1/2 in FT194 cells induces expression of FGF1, FGF2, FGFR1, and FGFR4. d) Blockage of YAP and FGFR using verteporfin and BGJ398 inhibited FT194 cell proliferation induced by LATS1/2 knockdown. Each box represents Mean ± SEM of 4 independent experiments. Boxes with different letters are significantly different from each other. e) Blockage of YAP and FGFR using verteporfin and BGJ398 inhibited FT194 cell migration induced by LATS1/2 knockdown. BGJ: BGJ398 (1μM); VTPF: verteporfin (5μm). Scale bar=200μm.