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. 2015 Sep 14;35(20):2655–2663. doi: 10.1038/onc.2015.328

Figure 5.

Figure 5

LZTFL1 inhibits Hh signaling. (a) Relative SHH level in HCC95-GFP and HCC95-LZTFL1-GFP cells normalized against internal GAPDH. (b) Western blotting of cell lysates from HCC95-GFP and HCC95-LZTFL1-GFP cells probed with antibodies against SHH and LZTFL1. GAPDH was used as a loading control. (c) Gli1-luc reporter activities from Hh reporter cell line light2 cultured alone, with HCC95-GFP or with HCC95-LZTFL1-GFP cells (n=3±s.e.m., *P<0.05, LZTFL1 vs GFP). (d) IHC of SHH from sections of HCC95-GFP and HCC95-LZTFL1-GFP tumors grown in ex vivo lung culture.