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. 2015 Nov 2;6(41):43496–43507. doi: 10.18632/oncotarget.6292

Figure 4. Ad.4N1-IL24 elicited higher cytotoxicity to leukemia cells in vitro.

Figure 4

a. Schematic structure of Ad.4N1-IL24. Viral E1B was deleted (dE1B), and the resulted restriction site was used to harbor the gene encoding IL-24. b. K562 or c. HL60 cells were treated with Ad.4N1 or Ad.4N1-IL24 at MOIs indicated for 72 h. Cell viability was analyzed by MTT assay. Values were calculated as percent of PBS control and presented as mean ± SEM. (*p < 0.05) d. HL60 cells treated with Ad.4N1 or Ad.4N1-IL24 at 200 MOI for 48 h were analyzed by Western blot for levels of Caspase 3, Bcl-2, and IL-24. GAPDH served as the loading control. e. HL60 cells were treated with Ad.4N1 or Ad.4N1-IL24 at 100 MOI. After 48 h or 72 h, cells were collected and intracellular viruses were released by 3 freeze-thawing cycles. Viral production was determined through TCID50 assay. Values were shown as mean ± SEM. (*p < 0.05)