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. 2015 Oct 19;6(41):43508–43528. doi: 10.18632/oncotarget.6180

Figure 10. Archazolid A-induced apoptosis in PDX is in line with decreased levels of the anti-apoptotic protein survivin.

Figure 10

A. Upper panels show apoptosis rates (specific cell death) determined by PI exclusion staining of PDX leukemia samples treated with Archazolid A (Arch, 10 nM, 48 h). Lower panels display immunoblots from PDX cells from the same patients treated with Archazolid A (Arch, 10 nM, 24 h) and probed with antibodies for survivin. Immunoblots for actin indicate equal loading. B. Archazolid A mediated cell death is partially rescued by survivin overexpression. The graph shows early apoptosis (AnnexinV-positive and PI-negative cells) determined by AnnexinV/PI staining of Jurkat cells overexpressing either empty vector (ev) or survivin and treated with/without Archazolid A (Arch) at indicated concentrations for 48 h. One-Way ANOVA, Tukey, *p ≤ 0.05, ***p ≤ 0.001, n = 3. Immunoblots show overexpression of empty vector (ev) and survivin 24h after transfection; actin indicates equal loading.