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. 2015 Oct 19;6(41):43508–43528. doi: 10.18632/oncotarget.6180

Figure 6. NICD cannot rescue Archazolid A mediated induction of apoptosis.

Figure 6

A. Apoptosis rate determined by Nicoletti assay (left bar graph) and by Annexin V/PI staining (right bar graph) of Jurkat cells overexpressing either empty vector or NICD after treatment with Archazolid A (48h) is shown. Immunoblots show NICD overexpression. n = 3. B. Increased expression of Notch downstream targets Hey1 (paired t-test, *p = 0.0197), Hey2 (paired t-test, p = 0.0576), NRARP (paired t-test, *p = 0.0407), Hes1 (paired t-test, p = 0.1117) after NICD overexpression (24h) is shown. n = 3. C. Proliferation of Jurkat cells overexpressing either empty vector or NICD after treatment with DBZ at indicated concentrations for 72h is shown. t-test, *p = 0.0209, n = 3. D. Expression of V-ATPase subunit c (ATP6V0C) of Jurkat cells treated with DBZ (50 μM, 24h) is shown. Non-significant (ns), paired t-test, p = 0.1886.