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. 2015 Oct 20;6(41):43540–43556. doi: 10.18632/oncotarget.6183

Figure 1. SOX2 is expressed in BC and promotes clonogenicity.

Figure 1

(A) Endogenous SOX2 mRNA (left) and protein (right) expression in BC cell lines MCF7, BT474, and T47D propagated under standard (2D) cultivation conditions. Indicated are mRNA expression levels relative to GAPDH. Midline illustrates average SOX2 expression in the three cell lines analyzed. Actin is shown as a protein loading control. (B) Relative SOX2 mRNA expression in 10 primary patient-derived BC samples (P1 and P2: samples showing highest endogenous SOX2 expression, midline to illustrate average). (C) Reduced SOX2 mRNA and protein expression, and (D) impaired sphere formation in MCF7 cells transduced with SOX2 shRNA vs. control lentiviral particles. (E) Inducible mCherry-SOX2 expression in stably transfected T47D cells at 24 hours of induction with 1 μg/ml of doxycycline, as verified by qRT-PCR (left) and immunoblotting (right). (F) Ectopic expression of a mCherry-SOX2 fusion protein (SOX2 OE) induces sphere formation in T47D cells. Samples were incubated in 3D medium in the absence or presence of doxycycline (1 μg/ml) for 5 days.