Table 1. Parameters for anchor insertion depending on lipid composition and anchor type.
Lipid composition | Anchor type | Insertion time | Insertion temperature (°C) | Method |
---|---|---|---|---|
SPC/Chol | Chol-PEG2000-NHS | 30 | 17 | SPIT |
DSPE-PEG2000-NHS | 60 | 60 | PIT | |
HSPC/Chol/DSPE-mPEG, Caelyx® | Chol-PEG2000-NHS | 60 | 20 | SPIT |
60 | 60 | SPIT60 | ||
DSPE-PEG2000-NHS | 60 | 60 | PIT |