Skip to main content
. 2015 Nov 3;6(41):43911–43926. doi: 10.18632/oncotarget.6084

Figure 6. Knockdown of JNK or c-jun decreases GA-induced autophagy in A549 and NCI-H1299 cells.

Figure 6

A–B. A549 and NCI-H1299 cells were transiently transfected JNK siRNA, cultured for another 24 h, and treated with GA (40 μM) for 24 h. Western blot analysis was used to evaluate protein expression. *P < 0.05 and **P < 0.01. C. A549 cells were transiently transfected with GFP-LC3 for 24 h and JNK siRNA for 24 h. GA (40 μM) was added to the cells, and culture for another 24 h was performed. GFP-LC3 punta were examined using IN cell Analyzer 2000, and typical images were shown. D–E. After knockdown of c-jun by c-jun siRNA, A549 and NCI-H1299 cells were treated with GA (40 μM) for 30 min or 24 h. Western blot assay was used to detect changes in protein levels. P < 0.05 and **P < 0.01.