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. 2015 Nov 3;6(41):43911–43926. doi: 10.18632/oncotarget.6084

Figure 7. The IRE1α-JNK/c-jun pathway is essential for GA-induced autophagy in A549 and NCI-H1299 cells.

Figure 7

A. A549 cells were treated with varying concentrations of GA for 24 h, and the ER stress-related protein expression was evaluated by Western blot analysis. B. A549 cells were transiently transfected with GFP-LC3 and IRE1α siRNA for 24 h. Cells were treated with GA (40 μM) for 24 h. GFP-LC3 punta were evaluated by IN Cell Analyzer 2000, and typical images were shown. C–D. A549 and NCI-H1299 cells were infected with scramble or IRE1α siRNA and then incubated for 24 h. GA (40 μM) was added to the cells for 30 min or 24 h. Protein expression related to the JNK/c-jun pathway and autophagy was detected by Western blot analysis. P < 0.05 and **P < 0.01.