Table 1.
Optimized tuning conditions for analysis of methylpyridinium derivatives of estrogens.
Analyte | Precursor ionm/z | Product ionsm/z | Collision energy (V) | Collision cell exit potential (V) | De-clustering potential (V) | Entrance potential (V) |
---|---|---|---|---|---|---|
E1 | 362 | 238* | 57 | 10 | 136 | 10 |
252$ | 57 | 10 | ||||
E2 | 364 | 128* | 19 | 18 | 51 | 10 |
110$ | 39 | 10 | ||||
13C2E1 | 364 | 240* | 55 | 10 | 61 | 10 |
254$ | 57 | 12 | ||||
13C2E2 | 366 | 130* | 63 | 28 | 76 | 10 |
110$ | 59 | 12 | ||||
d4E1 | 366 | 240* | 59 | 22 | 81 | 10 |
254$ | 57 | 24 | ||||
d4E2 | 368 | 130* | 39 | 14 | 56 | 10 |
111$ | 23 | 10 |
Voltage (V); estrone (E1); estradiol (E2); 3,4-[13C]2-estrone (13C2E1); 2,4,16,16-[2H]4-estrone (d4E1); 3,4-[13C]2-estradiol (13C2E2); 2,4,16,16-[2H]4-estradiol (d4E2); quantifier (*) and qualifier ($).