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. 2016 Mar 15;7:93. doi: 10.3389/fphys.2016.00093

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Copyright © 2016 Prathipati, Metreveli, Nandi, Tyagi and Mishra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion of MMP9 gene increased the levels of SERCA-2a in diabetic hearts. (A) QPCR showing the mRNA levels of SERCA-2a in WT, Akita, and DKO hearts. Values are mean ± SEM. N = 5, ^*P < 0.05. (B) Top, representative Western blot bands for SERCA-2a and GAPDH (a loading control). Bottom, bar graph showing densitometric analyses of SERCA-2a band intensity. N = 3. Values are mean ± SEM. ^*P < 0.05. (C) Top, representative immunoprecipitation bands for SERCA-2a. Bar graphs showing relative expression of SERCA-2a in the three groups. Bottom, bar graph showing the relative values of densitometric band intensity (arbitrary unit, a. u.) N = 2.