Figure 1.

Stomatal Opening Is Associated with a Reduction in Abundance of LDs, and This Response Involves the Blue Light Receptors PHOT1 and PHOT2

(A) Guard cells contain cytoplasmic NR-staining LDs (i, bright field; ii, autofluorescence; iii, NR fluorescence; iv, overlay of ii and iii; scale bar, 5 μm).

(B) Light-induced stomatal opening is associated with a decrease in NR fluorescence (n = 120 for each; p < 0.001 at 4 hr for both; error bars represent ±SE).

(C) Light-induced stomatal opening is disrupted in the phot1/phot2 double mutant, as is LD breakdown as estimated by NR fluorescence (n = 90 for aperture; n = 75–95 for volume; p < 0.001 at 2 hr and 4 hr for stomatal opening and p < 0.05 for LD reduction).

See also Figure S1.