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. 2016 Mar 12;3(2):ENEURO.0031-16.2016. doi: 10.1523/ENEURO.0031-16.2016

Figure 1.

Figure 1.

Bruchpilot distribution is disrupted in rab3-GEF mutant NMJs. A, Schematic representations of the cloned WT Drosophila rab3-GEF gene, the rab3-GEFMA18 and rab3-GEFMA20 mutant genes generated by EMS mutagenesis, and the rab3-GEFSC225 mutant gene generated by P-element excision. Magenta exonic regions correspond to the tripartite DENN domain composed of the uDENN, DENN, and dDENN modules. The green exonic region corresponds to the DD. Arrowheads identify the Q1862STOP and G460E point mutations associated with the rab3-GEFMA18 and rab3-GEFMA20 mutant alleles, respectively. Boxed region of the rab3-GEFSC225 mutant allele denotes the part of the gene that is excised. B, Confocal images of muscle 4 NMJs costained with antibodies against the presynaptic AZ protein Brp (magenta) and the postsynaptic receptor DGluRIII (green) from WT (CS), rab3-GEFMA18 mutant (rab3-GEFMA18/Df(1)ED7289) and rab3-GEFMA20 mutant (rab3-GEFMA20/Df(1)ED7289) third-instar larvae. Scale bar, 2 µm.