Table 1.
Primer used for CAP gene expression in ATCC and clinical C. neoformans.
| Gene | Primer sequence (5′–3′) | Product size | Specification | Reference | |
|---|---|---|---|---|---|
| CAP10 | GGATGCAGAGTTGAGGAAGA | F | 154 | Glycosyltransferase | Okabayashi et al., 2005 |
| TATCCGTTGTAGGTCATGGC | R | ||||
| CAP59 | CCGAACGAAGAAATCTCACT | F | 244 | Encodes to transmembrane protein | |
| CTAGGTTGCATGTGTTCCCA | R | ||||
| CAP60 | TCATGAGGGTGGAAACTACA | F | 221 | Unknown | |
| GGGATGGATGAGTCTGAAAT | R | ||||
| CAP64 | ATTGACTTTGATCGACGAGA | F | 164 | Gene regulation | |
| ACTCTTCCTCGATCAATGTC | R | ||||
| β-actin | GCCCTTGCTCCTTCTTCTAT | F | 156 | Housekeeping gene | |
| GACGATTGAGGGACCAGACT | R | ||||
| DNA ladder | Gene Ruler 1Kb DNA Ladder (SM0311) | 250–10000 bp | |||
F, forward primer; R, reverse primer.