Figure 7. Cholesterol/CD supplementation restores plasma membrane cholesterol levels and suppresses the antiviral activity of ICZ against HRV1B.

HRV1B-uninfected (A) or -infected (B) HeLa cells were treated with vehicle or 10 μm ICZ, and HRV1B-infected cells were supplemented with cholesterol/CD (20 μg/ml cholesterol in 5 mg/ml CD) after ICZ treatment. Cells were fixed and stained for the cholesterol marker filipin III (green) and probed for the endosome marker LAMP-1 (red). Scale bar, 20 μm. (C) The percentage of cholesterol in plasma membrane versus cytosol was calculated from (B).