Aβ attenuated microglial IGF-1 secretion but IGF-1 did not alter Aβ-mediated TNFα secretion from microglia. A, In order to determine effects of microglial stimulation on IGF-1 secretion C57BL/6 microglia were stimulated with or without 1μM Aβ 1-42 or 2.5 ng/mL LPS overnight and media collected for IGF-1 ELISA. B, Cell viability was assessed via MTT reduction (560/650nm). Data is from 4 individual cultures in each group and displayed as mean +/− SD, *p<0.05. C, To evaluate the effect of IGF-1 on Aβ stimulation, C57BL/6 microglia were stimulated overnight with or without 1μM Aβ 1-42 and 100ng/mL IGF-1. Data is from 4 individual cultures in each group and displayed as mean +/− SD, *p<0.05.