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. 2016 Apr 1;39(4):743–755. doi: 10.5665/sleep.5620

Figure 4.

Figure 4

DNA methylation and protein expression levels of the NPR2 and SP140 genes in the validation cohort. (A) DNA methylation levels over both −608 CpG site of the NPR2 promoter region were decreased, (B) whereas NPR2 protein expression levels were increased in patients with sleep disordered breathing (SDB) with excessive daytime sleepiness (EDS) (Epworth Sleepiness Scale [ESS] score > 10) as compared to that in those without EDS. (C) DNA methylation level over −608 CpG site of the NPR2 gene was negatively correlated with ESS. (D) Serum CNP levels were increased in patients with SDB with EDS as compared to that in those without EDS. (E) Serum CNP was negatively correlated with DNA methylation level over −618 CpG site of the NPR2 gene. (F) DNA methylation levels over −194 CpG site of the SP140 promoter region in patients with SDB with EDS were increased, (G) whereas SP140 protein expression levels were decreased in patients with SDB with EDS (ESS > 10) as compared to those without EDS. (H) DNA methylation level over −194 CpG site of the SP140 gene was positively correlated with ESS. *Compared by independent Student t-test. #Adjusted by multivariate linear regression model.