Skip to main content
. 2016 Apr 1;39(4):861–874. doi: 10.5665/sleep.5644

Figure 3.

Figure 3

Effects of sleep and wake on the endosomal compartments. (A) Left panel: example of an early endosome (EE); right panel: example of a multivesicular body (MVB). (B) Box plot representing the density of EEs across experimental groups (99 in S, 113 in ASD, 67 in CSR, and 117 in RS). (C) Cumulative distribution of the percentage of cytoplasm occupied by EEs across groups. (D) Density of EEs (white bars) and MVBs (black bars) across groups. (E) Left: schematic illustration of invagination and back-fusion events: MVB biogenesis and formation of internal vesicles occurs by invagination of MVB's outer membrane and incorporation of receptors and lipids coming from the plasma membrane; during back-fusion, internal vesicles fuse with the MVB's outer membrane and then bud off to bring recycled material to the plasma membrane. Right: micrographs with examples of invagination and back-fusion in MVBs and EEs. (F) Percentage of total endosomal compartments undergoing either invagination or back-fusion across all conditions. CSR, chronic sleep restriction; RS, recovery sleep; S, sleep; ASD, acute sleep deprivation. Scale bar for all micrographs = 0.5 μm. Bars indicate mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.