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. 2016 Mar 15;6:22957. doi: 10.1038/srep22957

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Copyright © 2016, Macmillan Publishers Limited

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A chamber was used to create a defined open area in between the AdMSCs. The cells were cultured in constant CSE concentrations (0, 0.5, 1, 5, and 10%) after the chamber was removed and were stained with Calcein AM (green) to visualize live cells. After 24 h, migration of the cells was evaluated. Control cells and those exposed to 0.5 and 1% CSE exhibited continual migration through 48 h. The cells exposed to 5% CSE showed very limited migration throughout the experiment and those exposed to 10% CSE scarcely survived.