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. 2016 Mar 15;6:22957. doi: 10.1038/srep22957

Figure 4. Lipid vacuole formation and gene expression for adipogenic differentiation.

Figure 4

AdMSCs were allowed to reach confluency and were then exposed to 0.5% CSE at the moment of induction of adipogenic differentiation with supplemented medium and for 24 h after induction. (A) After 21 d in culture, the cells were stained with Oil Red O to detect lipid vacuoles (10× magnification). (B) The Oil Red O solution was eluted and the optical density was measured, indicating there were no significant differences in lipid vacuole formation after acute CSE exposure. (C) RNA was extracted from the cells and the gene expression of early (PPARγ), late (LEP), and abundant (ADIPOQ) markers was analysed with qPCR, revealing no significant differences after 3 d.