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. 2016 Mar 15;6:23188. doi: 10.1038/srep23188

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(A) Representative Western blot analysis (top) and mean data obtained by densitometry analysis (bottom) are shown. GAPDH served as loading control. The bars indicate mean ± SD. ANOVA was performed to evaluate statistical significance of differences, n = 6, **P < 0.01. (B) Representative immunohistochemistry in different age groups. Scale bar: Left panel, 100 μm; Right panel, 20 μm. (C) Representative immunofluorescence in different age group. Scale bar: 25 μm.