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. 2016 Mar 15;9:29. doi: 10.1186/s13041-016-0209-3

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© Mardones et al. 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — FZD1 is expressed in NSCs, amplifying progenitors and immature neurons of the adult mouse hippocampus. a Representative immunodetection of FZD1, SOX2 and GFAP in the dentate gyrus of 2-month-old mouse. Scale bar: 50 μm. Right, higher magnification of the image. Arrows indicate SOX2-positive cells with a single GFAP-positive projection. Arrowhead indicates a cell only positive for SOX2. Scale bar: 5 μm. b Immunodetection of FZD1, GFAP and the mitotic marker Ki67, scale bar: 50 μm. Right, higher magnification of the image. Arrows indicate cells positive for FZD1 and Ki67 staining. Scale bar: 10 μm. c Representative immunodetection of FZD1, DCX and NeuN in the dentate gyrus of 2-month-old mouse. Scale bar: 50 μm. d Higher magnification of the image shown in c. For simplification, only the double staining FZD1/DCX is shown. Arrows indicate FZD1-staining in the cell body of DCX-positive immature neurons. Scale bar: 10 μm. ML: molecular layer, GCL: granule cell layer, SGZ: subgranular zone, H: hilus