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. 2016 Mar 15;9:29. doi: 10.1186/s13041-016-0209-3

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© Mardones et al. 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — FZD1 is expressed in AHPs isolated from adult mouse hippocampus. a Monolayer of AHPs isolated from 6-week-old mouse hippocampus and cultured under proliferation conditions. Scale bar: 50 μm. b Immunoflurescence staining of SOX2 and nestin in AHPs. Nuclei were stained with NucBlue (NucB). Scale bar: 10 μm. c RT-PCR analysis of the expression of FZD1 and GAPDH in cultured AHPs (lane 1), N2a cells (lane 2), mouse hippocampus used as positive control (lane 3) and water used as PCR negative control (N/C, lane 4). d Immunoflurescence staining of FZD1, nestin and SOX2 in AHPs. Scale bar: 50 μm. Bottom panels, higher magnification of the cells indicated in top panels. Scale bar: 20 μm