Fig. 5.

FZD1 knockdown affects migration but not dendritic development of newborn neurons. a Immunofluorescence staining of NeuN in animals sacrificed 4 wpi. Scale bar: 30 μm. Right panels, single focal planes of merged images (ZsG/NeuN) or NeuN staining separated. Dotted circles indicate the neuron that is shown in right panels. b The percentage of migration of ZsG + NeuN+ cells into the GCL was evaluated as shown in the sketch. The graph shows the migration of 4-week-old ZsG + NeuN+ cells. ***p < 0.001, Student’s t-test. c Denditric development of 4-week-old ZsG+ neurons. Images show 2D reconstructions of neurons infected with shC (top panel) and shFZD1 (bottom panel). Insets show representative NeuN-staining in ZsG+ cells. d, e Total dendritic length (d), and total number of intersections (e) of 4-week-old ZsG+ neurons. N ≥5 neurons; ns: not significant, Student’s t-test, p = 0.141 (d), p = 0.163 (E). f Sholl analysis of dendritic complexity of ZsG + NeuN+ cells. ML, molecular layer; GCL, granule cell layer; H, hilus