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. 2016 Mar 15;15:159. doi: 10.1186/s12936-016-1226-5

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© Burns Jr. et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Specificity of antibody response induced by immunization with rPfMSP8 (ΔAsn/Asp) versus rPfMSP1/8 vaccines. Antigen-specific IgG titres (mean ± standard deviation) in sera collected from Aotus monkeys (6 animals/group) immunized with a rPfMSP8 (ΔAsn/Asp) or b rPfMSP1/8 vaccines formulated with Montanide ISA 720 were determined by ELISA. Sera collected 2 weeks following primary, secondary and tertiary immunization were evaluated. ELISA plates were coated with either rPfMSP1/8, rPfMSP8 (ΔAsn/Asp), rGST-PfMSP1₁₉ (FVO), or rGST-PfMSP1₁₉ (3D7) as indicated. For each animal, reactivity of pre-immunization serum was subtracted as background