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. 2016 Mar 15;6:10. doi: 10.1186/s13601-016-0098-7

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© Santos and Lack. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Diagram of the laboratory procedure for the basophil activation test. Following stimulation of blood cells with allergen or controls, blood cells are stained with antibodies coupled to a fluorochrome, which allow the identification of cells and the measurement of the expression of activation markers using a flow cytometer