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. 2016 Mar 14;16:215. doi: 10.1186/s12885-016-2231-3

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© Peralta-Zaragoza et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Detection of caspases activity in response to miR-21 silencing. SiHa (panel a) and HeLa (panel b) cells were analyzed by caspase-3/7 activities, 48 h after of non-treated (lane NT), treated with H₂O₂ (apoptosis control), transfected with empty vector (pSilencer 1.0-U6) and transfected with pSIMIR21 plasmids. DMEM medium alone was included. Following transfection, luciferase activity levels were measured and luminescence value corresponded to relative light units. The values are presented as mean ± SD and P values <0.01 are indicated with asterisks. Each assay was carried out three separate times