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. 2016 Mar 15;14:71. doi: 10.1186/s12967-016-0830-z

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© Vandsemb et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression of PRL-3 in prostate cancer cell lines PC3 and DU145, which have an increased copy number of PRL-3 gene locus. a Prostate cancer cell lines PC3 and DU145 were analyzed for PRL-3 expression by quantitative RT-PCR. PRL-3 transcript levels were normalized to GAPDH expression in each sample and presented as relative to the PRL-3 expression in the PC3 cell line, which was set to one. Mean CT values are presented in the bars. b PRL-3 protein expression was determined by Western blotting. The INA-6 cell line was used as positive control. GAPDH was used as loading control. c, d Fluorescence in situ hybridization with whole chromosome and PRL-3 probe on prostate cancer cell lines PC3 (c) and DU145 (d). The red signal marks PRL-3 (8q24.3) and the green signal marks the whole chromosome 8