Fig. 8.

Identification of a multi-functional miRNA precursor of miR168 in pear winter buds. a The secondary structure of the miR168 precursor. The regions producing miR168.1 and miR168.2 are indicated. b Abundances of miR168.1, miR168.2 and their star sequences produced from the precursor. Endo2009 was used as the example. c The binding sites of miR168.1 and miR168.2 on two target transcripts. d T-plot indicated the cleavage events mediated by miR168.1 and miR168.2 based on the PARE-seq. e Cleavage sites mediated by miR168.1 and miR168.2. The RML-RACE were applied to confirm the cleavage events identified with PARE-seq