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. 2016 Mar 14;17:124. doi: 10.1186/s12891-016-0979-x

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© van den Akker et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — EGR1 expression is increased in degenerate NP cells. a Immunodetection of EGR1-positive cells in healthy control NP tissue (L1/L2 IVD of a 63 years old donor; left panel) and in degenerate NP tissue (L4/L5 IVD of the same donor middle panel); right panel: staining control on degenerate NP section: primary antiserum detecting EGR1 was left out. b Quantification of EGR+ cell numbers in healthy or degenerate NP tissue. Cell numbers are depicted as percentage of total cell number scored (total cell counts: 121 for healthy tissue and 218 for degenerate tissue)