Fig. 6.

Identification of genes bound and regulated by p38α. a, b The two data sets (expression array and ChIP-on-chip) were crossed to identify genes bound and regulated by p38α. The non-proportional 2-way Venn diagrams show that, at 24 h of differentiation (DM), p38α binds to the promoter of 46 genes upregulated in SB-treated C2C12 cells (a) and to 70 genes downregulated in myoblasts lacking active p38α (b). Only 10 and 12 of these bound genes are also upregulated and downregulated, respectively, in p38α-deficient satellite cells. c Gene ontology analysis of genes bound and regulated by p38α in C2C12 cells at 24 h in DM. Enriched GO annotations of genes downregulated in SB-treated C2C12 myoblasts are shown in green whereas enriched GO annotations of upregulated genes are shown in red. d DAVID analysis of canonical pathways enriched in genes bound by p38α showed that Wnt signaling pathway was one of the main signaling modules regulated by p38α. KEGG pathway is shown with p38α-bound promoters highlighted