Table 4.
Significant functional terms found for the genes displaying an increase in DNA methylation and expression at d7 relative to d1 in neonatal murine hearts
| GOID | GOTerm | p-value corrected with Bonferroni step down | Associated Genes Found |
|---|---|---|---|
| GO:0098742 | cell-cell adhesion via plasma-membrane adhesion molecules | 8.09E-04 | Cdh1, Cdh13, Cdh26, Esam, Igsf9, Pcdhga12, Scarf2, Umod |
| GO:0048705 | skeletal system morphogenesis | 1.43E-03 | Alpl, Bmp4 , Chad, Hoxa3, Hoxa5, Hoxb5, Ltbp3, Osr1 , Tcf15, Tgfb3 |
| GO:0009952 | anterior/posterior pattern specification | 5.37E-03 | Bmp4 , Hoxa3, Hoxa5, Hoxb5, Med12, Meox1, Osr1 , Tcf15, Zic3 |
| GO:0031032 | actomyosin structure organization | 1.01E-02 | Ankrd23, Asap3, Lmod2 , Myh7b, Mypn , Neb , Tpm1 |
| GO:0048771 | tissue remodelling | 1.68E-02 | Clec10a, Dll4 , Hoxa3, Ltbp3, Nol3, Pml, Spp2 |
| GO:0055002 | striated muscle cell development | 3.74E-02 | Ankrd23, Bmp4, Lmod2, Mypn, Neb , P2rx2 |
| GO:0045064 | T-helper 2 cell differentiation | 8.02E-03 | Bcl3, Hlx, Irf1 |
| GO:0005520 | insulin-like growth factor binding | 3.24E-02 | Cyr61, Htra4, Igfbp6 |
As revealed by ClueGo gene ontology analysis, a group of 215 genes showing a gain in DNA methylation and a minimum 1.5-fold increase in expression level was significantly enriched in developmental genes. Remarkable genes reported to participate in heart functions a development are distinguished by bold font. (The 215 genes are are listed in Additional file 1: F1 quadrant of Fig. 8)