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. 2016 Mar 14;212(6):707–719. doi: 10.1083/jcb.201507054

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© 2016 Le et al.

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Figure 1. — SLIT2/ROBO1 regulates actin organization in the mammary gland and epithelial contraction of compliant matrices. (A) Grazing sections of Robo1^+/+ and Robo1^−/− murine mammary glands stained with Cytokeratin-14 (CK-14; red), phalloidin (green), and Hoechst (blue). Bars, 30 µm. (B and C) Quantification of fiber density (B) and fiber thickness (C) in grazing sections (n = 3 pairs of animals). (D) Cartoon depicting cell contraction in LD, 3D collagen gels. (E, H, J, and L) Representative micrographs of collagen gels containing (E) primary MECs, (H and J) NMuMG cells, and (L) MDA–MB-231 cells. Dashed outline represents measured area. (F, G, I, K, and M) Quantification of the percentage reduction in collagen gel area for gels embedded with Robo1^+/+ and Robo1^−/− MECs (F and G), NMuMG cells treated with SLIT2 (I and K), and MDA–MB-231 cells infected with Robo1 or scrambled (SCR) shRNA lentivirus (M) (n = 3 experiments). Asterisks denote significance by t test: *, P < 0.05; ***, P < 0.001.