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. 2016 Mar 14;212(6):707–719. doi: 10.1083/jcb.201507054

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© 2016 Le et al.

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Figure 2. — SLIT2/ROBO1 signaling promotes Rac-mediated cell contraction of compliant matrices. (A, top) Representative blots for GTP-Rac, total Rac, and GAPDH. (bottom) Quantification of Rac activation (n = 3 experiments). (B, D, and F) Images of 3D LD gels with embedded NMuMG cells subjected to indicated treatments: Mock, SLIT2, NSC23766 (NSC), Sos1 or control siRNA, Y27632 (ROCK Inhibitor), or C3-Transferase (Rho inhibitor, C3-Trans). Dashed outline of gels represent measured areas. (C, E, and G) Quantification of the percentage reduction in collagen gel area for treated gels (n = 3 experiments). (H and J) Representative blots of pMRLC19, MRLC, and GAPDH from NMuMG cells treated with Mock or SLIT2 and indicated siRNA and inhibitors. (I and K) Quantification of pMRLC normalized to total MRLC and GAPDH (n = 3 experiments). Asterisks denote significance by t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. ns, not significant.