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. 2016 Mar 14;212(6):633–646. doi: 10.1083/jcb.201508068

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© 2016 Randise-Hinchliff et al.

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Figure 5. — Opi1 and Ume6 are sufficient to block GRS I and GRS II zip code function, but not PRE zip code function. (A, top) Schematic of the INO1 promoter having a LexA BS in the ino2 L118A strain (which blocks the interaction of Ino2 with Opi1; indicated with an X). (bottom) Peripheral localization of ino2 L118A LexA BS-INO1 in strains expressing LexA, LexA-Opi1, or LexA-Ume6 grown under the uninducing condition. (B, top) Schematic of LacO128-LexA BS ± zip codes integrated at URA3. The spacing from the middle of the LexA BS to the middle of the zip codes was GRS I = 68 bp, GRS II = 58 bp, 3×PRE = 42 bp. (bottom) Peripheral localization of URA3-LexA BS ± the indicated zip codes in strains expressing LexA, LexA-Opi1, or LexA-Ume6. For A and B, the color indicates the p-value from the Fisher exact test, compared with the control. (C) Distribution of distances between INO1 and URA3-GRS I-LexA BS in strains expressing LexA or LexA-Opi1, grown under inositol starvation. (top) Mean distances and standard deviations. P-value from Wilcoxon rank sum test comparing the distributions. (D) Fraction of cells in which INO1 and URA3-GRS I-LexA BS were ≤0.55 µm apart (p-values from Fisher exact test).