Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 14;212(6):633–646. doi: 10.1083/jcb.201508068

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Randise-Hinchliff et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — A general role for repressors in regulating zip code–mediated targeting to the nuclear periphery. (A and B) Peripheral localization of URA3-GRS I-LexA BS (A) or URA3-GRS I (B) in strains expressing LexA alone (−) or LexA fused to the indicated full-length repressors (see www.yeastgenome.org for descriptions of all gene names). These LexA fusions were validated as described in Methods and in Fig. S3. LexA fusions were tested for their ability to block GRS I–mediated targeting in both RPD3 (A and B; light green or forest green) and rpd3 H188A (A; lavender or purple) strains. Repressors that resulted in a statistically significantly decrease in peripheral localization compared with LexA alone (P < 0.05; Fisher exact test) are indicated in light green (RPD3) and lavender (rpd3 H188A); repressors that did not affect GRS I–mediated peripheral localization are indicated in forest green (RPD3) and purple (rpd3 H188A) bars. Mean and SEM from three of more biological replicates (30–50 cells per replicate).