Figure 7.

MAPK phosphorylation of Dig2 regulates Ste12-mediated peripheral targeting and interchromosomal clustering. (A) Schematic for regulation of Ste12-dependent transcriptional activation of PRM1 by Dig1 and Dig2. (B) Peripheral localization of the PRM1 gene in wild-type (WT) and mutant strains ± α-factor. Point mutations in Dig2 were introduced into the chromosomal DIG2 locus. (C, top) Schematic of URA3-LexA BS. (bottom) Peripheral localization of URA3-LexA BS in wild-type and dig2Δ strains expressing LexA or LexA-Ste12, grown under uninducing and inducing conditions. Mean and SEM from three of more biological replicates (30–50 cells per replicate); *, P ≤ 0.05 (Fisher exact test) between the uninducing and inducing condition. NS, not significant. (D) Distribution of distances in 0.2-µm bins between PRM1 and URA3:3×PRE in wild-type, dig1Δ, or dig2Δ MATa haploid strains ± α-factor. (top) Mean distances and standard deviations. (inset) P-values from Wilcoxon rank sum test for each comparison. (E) Fraction of cells in which PRM1 and URA3:3×PRE were ≤0.55 µm apart (p-values from Fisher exact test).