Table 4.
Validation of SSp. with 333 ticks
| Tick species name | N | True ID assigneda | No ID assigneda | Wrong ID assigneda | Sensitivity | Specificity | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| 4× CC | 3× CC 1× N | 2× CC 2× N | 3× CC 1× C | other | ||||||
| Amblyomma gemma | 21 | 4 | 4 | 5 | 0 | 4 | 4 | 0 | 81.00 % | 100.00 % |
| Amblyomma variegatum | 36 | 16 | 9 | 4 | 4 | 3 | 0 | 0 | 100.00 % | 100.00 % |
| Hyalomma dromedarii | 15 | 11 | 1 | 0 | 1 | 0 | 2 | 0 | 86.70 % | 100.00 % |
| Hyalomma marginatum rufipes | 14 | 4 | 4 | 2 | 0 | 2 | 2 | 0 | 85.70 % | 100.00 % |
| Hyalomma truncatum | 10 | 2 | 4 | 2 | 1 | 0 | 1 | 0 | 90.00 % | 100.00 % |
| Rhipicephalus (Boophilus) decoloratus | 37 | 25 | 7 | 1 | 2 | 2 | 0 | 0 | 100.00 % | 100.00 % |
| Rhipicephalus (Boophilus) microplus | 20 | 6 | 8 | 0 | 3 | 3 | 0 | 0 | 100.00 % | 100.00 % |
| Rhipicephalus appendiculatus | 74 | 53 | 13 | 3 | 2 | 2 | 1 | 0 | 98.60 % | 100.00 % |
| Rhipicephalus appendiculatus (Kiambu) | 5 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 80.00 % | 100.00 % |
| Rhipicephalus evertsi evertsi | 51 | 34 | 9 | 1 | 4 | 1 | 1 | 1 | 98.00 % | 98.00 % |
| Rhipicephalus pulchellus | 33 | 15 | 5 | 4 | 2 | 7 | 0 | 0 | 100.00 % | 100.00 % |
| Rhipicephalus simus group | 17 | 8 | 2 | 1 | 0 | 5 | 1 | 0 | 94.10 % | 100.00 % |
| 182 | 66 | 23 | 19 | 29 | ||||||
| Total | 333 | 319 | 13 | 1 | 96.10 % | 99.70 % | ||||
aFor each tick, four technical replicate mass spectra were matched against designed SSp. and a final ID assigned accordingly
CC: one correct SSp. matching; C: multiple SSp. matching, correct SSp. as top match; N: no matching SSp
other: true ID was assigned based on a different combination