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. 2016 Mar 15;6:23047. doi: 10.1038/srep23047

Figure 2. Rheological effects of catabolic and anabolic induction on hASCs with unconstrained morphology at subdiffusive loci (sDL) of the pnCSK.

Figure 2

(a) Examples of unconstrained hASC morphologies after 1-hr stimulation with catabolic [10 ng/ml rh-IL1α] and anabolic [10 ng/ml hPD-TGFβ1] cytokines. (b) Significant changes on geometric and rheological parameters describing altered hASC pnCSK mechanics after treatment with either rh-IL1α or hPD-TGFβ1. (c) Discriminant analysis of cytokine-induced cytoskeletal mechanics in hASCs with unconstrained shape in control media and following supplementation with rh-IL1α or hPD-TGFβ1. Error bars depict mean ± s.e.m interval (parameter effect significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Groups not sharing letters within a parameter are statistically significant (paired Tukey-Kramer post hoc: α < 0.05, >90% power). Receiver operating characteristic (ROC) curves and 50% density ellipses are specific to each independent group within experiments.