Figure 6. The double-antibody sandwich ELISA for the determination of B. cereus using rabbit polyclonal antibody (as capture antibody, 18 μg/mL) and monoclonal antibody 2D2 (as detecting antibody, 1:1000).

‘A’ is standard curve of sandwich ELISA. ‘B’ is linear detection line conserved from ‘A’, linear regression shows a working range from 1 × 10⁴ to 2.8 × 10⁶ cells/mL (r² = 0.993), as in the top left insert. X axle: the absorbance of microwell at 450 nm; Y axle: the log Kow of concentration of B. cereus.