Figure 2. Transgene expression following in vitro transfections.

16HBE14o- cells were transfected with minicircle DNA (green line) or plasmid DNA (red line) carrying the firefly luciferase gene (a) or the eGFP gene (b,c). For eGFP transfections both the percentage of positive cells (b) and the MFI (c) were assessed. ***P < 0.01; Student’s t-test used to assess significance. Values are background subtracted and displayed as mean ± SEM.