Figure 3. DNAH5 expression following in vitro minicircle and plasmid DNA transfections.

16HBE14o- cells were transfected with pMC.DNAH5 or MC.DNAH5 complexed into LED-1 particles and the relative amounts of DNAH5 (a) mRNA and (b) vector specific DNA quantified using qPCR and the 2- ΔΔCt method. NS, not significant; **P < 0.01; Student’s t-test used to assess significance. Values are mean ± SEM.