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. 2016 Mar 15;6:22389. doi: 10.1038/srep22389

Figure 3. Ewha-18278 inhibits RANKL-dependent ROS generation and OC differentiation.

Figure 3

Concentration (A) and time-dependent (B) Nox inhibition of Ewha-18278. BMMs were pretreated with indicated concentration of Ewha-18278 for 1 hr (A) or 10 μM Ewha-18278 for indicated times. After pretreated Ewha-18278, cells stimulated with RANKL (200 ng/ml) for 10 minutes. RANKL-induced ROS generation was monitored by confocal microscopic analysis of DCF fluorescence. Data represent three repeated experiments and are shown as mean ± SD (n = 3). (C) BMMs were treated with increasing concentrations of Ewha-18278 in the presence of M-CSF (30 ng/ml) and RANKL (100 ng/ml) and stained for TRAP activity. (D) TRAP+ MNCs containing more than 3 nuclei were counted. Data represent mean ± S.D., *P < 0.005. Scale bar, 500 μm.