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. 2016 Mar 15;6:22793. doi: 10.1038/srep22793

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(A) Bone-marrow-derived macrophages were isolated from healthy donor, based on CD68 expression using flow cytometry. (B) The isolated CD68+ macrophages were co-cultured with miR-454-modified PANC-1 cells in a transwell system for 48 hours. (C–D) The migrated macrophages into the lower panel were analyzed by flow cytometry based on CD68, shown by representative flow charts (C), and by quantification (D). *p < 0.05. N = 5.