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. 2016 Mar 15;3:160019. doi: 10.1038/sdata.2016.19

Figure 2. A representative example of signatures and quality of RRBS sequenced reads (sample: X9015).

Figure 2

(a) Per base sequence content as indicated by FastQC of the raw sequenced reads. The X-axis plots the sequencing cycle or positions in reads. The Y-axis represents percentages if the occurrence of the bases along the read. (b) Per base sequence content as indicated by FastQC after adaptor sequence removal. (c) A representative example of per base sequence quality for RRBS data (sample: X9015). For each position a Box and Whisker plot of the Phred quality scores is drawn. The central red line is the median value. The yellow box represents the inter-quartile range (25–75%). The upper and lower whiskers represent the 10 and 90% points. The blue line represents the mean quality.