Table 1. Overview of organellar proteome size predictions and selected proteomics approaches in Arabidopsis.

Note that for the predictor TPpred only the total number of 3194 Arabidopsis proteins with either chloroplast transit peptides (cTP) or mitochondrial transit peptides (mTP) was reported ⁶⁸.

Approach	(Estimated) number	Reference
chloroplast
cTP prediction (ChloroP) and correction for false positives/negatives	1900–2500	25
cTP prediction (TargetP)	3574	26
cTP prediction (TargetP)	3646	69
cTP prediction (TargetP) and correction for false positives/negatives	3130	31
cTP prediction (combination of predictors) and correction for false positives/negatives	2090	27
cTP prediction (Predotar)	1591	70
cTP prediction (TargetP)	4255	71
Mass spectrometry	690	72
Mass spectrometry + literature search	916	30
mitochondrion
mTP prediction (TargetP)	2897	26
mTP prediction (TargetP) and correction for false positives/negatives	3135	31
mTP predictions (combinations of predictors) and correction for false positives/negatives	2957	73
mass spectrometry	416	28
mTP predictions (combination of predictors)	2955–4514	28
mTP prediction (Predotar)	1105	70